Modified Liquid-Based Cytology Technique for Immunocytochemistry in Effusion Specimen
نویسندگان
چکیده
منابع مشابه
Improvization of conventional cytology by centrifuged liquid-based cytology in oral exfoliative cytology specimen
BACKGROUND Exfoliative cytology is the microscopic examination of shed or desquamated cells from the epithelial surface. Centrifuged liquid-based cytology (CLBC) is a modified technique that was used in the current study. AIMS To compare the efficacy of CLBC with conventional cytology in apparently normal mucosa after staining with Papanicolaou (PAP) stain. MATERIALS AND METHODS Fifty cases...
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BACKGROUND Laboratory detection of Human papillomavirus (HPV), Chlamydia trachomatis and Neisseria gonorrhoeae in liquid-based cervicovaginal cytology specimens is now based on identification of the DNA sequences unique to these infectious agents. However, current commercial test kits rely on nucleotide probe hybridization to determine DNA sequences, which may lead to diagnostic errors due to c...
متن کاملChromosome in situ hybridisation, Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology.
AIMS To assess the potential value of chromosome in situ hybridisation (CISH), Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology to help detect carcinoma cells and precursors. METHOD Sixty ThinPrep processed cervical cytology samples were studied: 23 cases within the normal limit, 13 low grade squamous intraepithelial lesions (LSILs), 10 high grade squamous intraepit...
متن کاملKi-67 immunocytochemistry in liquid based cervical cytology: useful as an adjunctive tool?
AIMS To test the ability of Ki-67 to detect cytological lesions in a screening setting and its use as a surrogate marker of human papillomavirus (HPV) infection. METHODS A study of liquid based cytology, HPV DNA testing by MY09/MY11 consensus polymerase chain reaction (PCR), type specific PCRs, and Ki-67 immunocytochemistry on a randomly selected series of 147 patients. RESULTS Comparison o...
متن کاملImmunocytochemistry and DNA-image Cytometry in Diagnostic Effusion Cytology. II. Diagnostic Accuracy in Equivocal Smears
To determine sensitivity and specificity of different antibodies for the immunocytochemical detection of malignant cells in diagnostically equivocal effusions in comparison with those achieved by DNA-image cytometry. 65 cytologically doubtful effusions of the serous cavities were stained with twelve antibodies. Furthermore, DNA-image cytometry was performed. Data were correlated with patient fo...
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ژورنال
عنوان ژورنال: Asian Pacific Journal of Cancer Prevention
سال: 2019
ISSN: 2476-762X
DOI: 10.31557/apjcp.2019.20.9.2611